Download Biomembranes Part R by Sidney Fleischer, Becca Fleischer (Eds.) PDF

By Sidney Fleischer, Becca Fleischer (Eds.)

The severely acclaimed laboratory usual, Methods in Enzymology, is likely one of the such a lot hugely revered courses within the box of biochemistry. considering 1955, each one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless appropriate this day - really a vital booklet for researchers in all fields of lifestyles sciences

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Grynkiewicz,M. Penie,and R. Y. Biol. Chem. 260, 3440 (1985). 28p. F. Bakerand D. E. Knight,Nature (London) 276, 620 (1978). 29H. Streb, R. F. Irvine,M. J. Merridge,and I. Schulz,Nature (London)306, 67 (1983). [ 1] TRANSPORT MACHINERY: AN OVERVIEW 11 opment of methods that were sufficiently sensitive to monitor uptake or release from the small fraction of volume occupied by vesicles in their bathing solution. A large variety of these methods exist, and combined application of dye and flux methods are often required to define the nature of transport at the vesicle level.

161, 611 (1986). 18 TRANSPORT THEORY [2] properties and eventually its molecular mechanism. The isolation of a specific membrane protein requires first its identification among the other membrane proteins. In some membranes the carrier protein is the major one and its identification is no problem. Normally, covalent or noncovalent labels to the carder are used for tracing the carrier. Also the functional assay in reconstituted vesicles is exploited for this purpose, when there is no other marker available.

Two groups of inhibitors are found, the atractyloside group and the bongkrekate group, which both interact with high selectivity only with the ADP/ATP carrier. 4,5,~4,15 The atractylosides interact only from the outside, whereas the bongkrekates bind only from the inside. Both types of inhibitors are assumed to bind to the substrate-binding center for ADP or ATP, although their structures are different. The only clear common denominator with the substrates is three or four negative charges. Because bongkrekate can penetrate the membranes unspecifically, it is particularly useful in inhibiting transport and is thus ideal for studying the compartmentalization of ADP and ATP within eukaryotic cells.

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