By Sidney Fleischer, Lester Packer
The severely acclaimed laboratory common, Methods in Enzymology, is among the such a lot hugely revered guides within the box of biochemistry. in view that 1955, every one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless suitable at the present time - really a necessary e-book for researchers in all fields of existence sciences
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Extra resources for Biomembranes Part B
Catalase crystals are a useful secondary standard 3s since their spacing has been determined by electron diffraction relative to gold 39 and the spacing remains unaltered by fixation with glutaraldehyde. 4° The limitations of the negative staining technique in determining the size of objects result from uncertainty as to the extent to which the specimen is penetrated by the stain and, where the layer of stain is relatively thick, uncertainty as to the extent to which portions of the specimen are masked (see Fig.
P. 71. Academic Press, New York, 1968. 5D. Deamer, R. Leonard, A. Tardieu, and D. Branton, Biochim. Biophys. Acta 219, 47 (1970). 46 CHARACTERIZATION OF MEMBRANES AND COMPONENTS  3. The fracture face should be shadowed and replicated immediately after the final fracture. Do not include an etching period unless further information may be obtained by sublimation of ice from the fracture face. Cleaning Replicas Replicas of pure lipid systems may be quickly cleaned in organic solvents--for instance, chloroform-methanol.
Staehelin) facilitates handling and minimizes the sample size needed. When copper or cellulose acetate disks are used, a grid pattern is scratched with a razor blade into the center two-thirds of the disk to provide better adhesion between sample and disk. Scratches should not extend to the disk's edge; if the sample flows to the edge, it may break off during attachment to the 11"Handbook of Chemistry and Physics" (C. , p. 2348. Chem. Rubber Publ. , Cleveland, Ohio, 1961-1962. n R. James and D.