By Willis A. Wood, Scott T. Kellogg
This quantity covers cellulose and hemicellulose and contains confirmed and reproducible equipment for study concerning the conversion of carbohydrate polymers to usable monomeric devices. Sections at the training of biomass fabrics and of substrates are integrated, as are sections on analytical tools and at the purification and assay of enzymes
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Extra resources for Biomass Part A: Cellulose and Hemicellulose
9 Separation of cellodextrins, which are homologous compounds of slightly different molecular dimensions, weight, and melting point (Table I), is a challenge. Microscale separation has been achieved by thin-layer chromatographic processes but larger scale separation preferentially uses liquid chromatography. Suitable chromatographic packings include charcoal and Celite, 2A3,14 charcoal and cellulose, 12 polyacrylamide and dextran, 2°-22 silica23,24DEAE-Spheron 300, 25 and cation-exchange resin in the Ca 2÷ form.
Chem. 10, 55 (1957). 13 G. L. Miller, J. Dean, and R. Blum, Arch. Biochem. Biophys. 91, 21 (1960). 14 G. L. Miller, Methods Carbohydr. Chem. 3, 134 (1963). 15 K. Hess and K. Dziengel, Ber. Dtsch. Chem. Ges. B 68, 1594 (1935). 16 E. E. Dickey and M. L. Wolfrom, J. Am. Chem. Soc. 71, 825 (1949). 17 M. L. Wolfrom and J. C. Dacons, J. Am. Chem. Soc. 74, 5331 (1952). 18 M. L. Wolfrom and A. Thompson, Methods Carbohydr. Chem. 3, 143 (1963). 19 M. Voloeh, M. R. Ladisch, M. Cantarella, and G. T. Tsao, Biotechnol.
Fukuyama, and T. Kuge, Carbohydr. Res. 121, 163 (1983). METHODS IN ENZYMOLOGY,VOL. 160 Copyright© 1988by AcademicPress, Inc. All rightsof reproductionin any formreserved. -. 6 eq 0 0 r-: - 28 CELLULOSE  having predominantly endocellulolytic activity are required and fractionation of large quantities of this cellulolytic complex is difficult. 12and later modified by Miller e t a l. 13,14Acetolysis followed by deacetylation is an alternative method. ~9 Separation of cellodextrins, which are homologous compounds of slightly different molecular dimensions, weight, and melting point (Table I), is a challenge.