By Lawrence S. Sturman (auth.), V. ter Meulen, S. Siddell, H. Wege (eds.)
This e-book is the results of a global symposium held on the Institute of Virology and Immunobiology of the-University of WUrzburg, Germany, in October 1980. The purpose of this symposium was once to supply a chance to check the information on coronavirus constitution and duplicate tion in addition to to debate mechanisms of pathogenesis. For over a decade coronaviruses were famous as a big staff of viruses that are chargeable for quite a few ailments of medical significance in animals and guy. lately new and fascinating information at the molecular biology and pathogenesis of coronaviruses became to be had and this led us to arrange this assembly. The uniformity and variety during this virus staff used to be evaluated from a molecular standpoint and the replication of coronaviruses seems to be to contain features that could be particular for this virus staff. also, not like different confident strand RNA viruses it grew to become transparent that coronaviruses quite simply identify chronic infections within the host, a phenomenon that could result in the various subacute or continual problems manifested in the course of coronavirus an infection. This quantity provides a sequence of articles dependent upon the medical presentation given on the symposium. additionally, there are articles by way of B. W. J. Mahy and D. A. J. Tyrrell which summarize the present country of paintings about the biochemistry and biology of coronaviruses, respective ly. We think this booklet should be of curiosity to all virologists and especially to either validated staff and rookies to this box. V.
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Additional info for Biochemistry and Biology of Coronaviruses
The staining patterns with Coomassie blue revealed a minimum of six bands, five of which might be considered major. One additional faint band was occasionally observed between the top two bands. 30 The molecular weights of the viral proteins were determined by co- and companion electrophoresis with proteins of known molecular weight (Table 1). Gels were also stained for lipid and carbohydrate. The lipid content of the single 1ipog1ycopolypeptide was more evident in gels in which the sample was not heated than in those in which it was heated at 100°C for 1 min.
The major 70S RNA of RSV is comprised of smaller fragments of variable size and linked by noncovalent bonds; a number of minor RNA components are also present. RNAs in each mixture, after preparation, were precipitated with ethanol in the presence of carrier RNA and analyzed in sucrose gradients. The profiles of acid-insoluble radioactivity for each gradient showed the same heterogeneity for the major class of OC-43 RNA fragments already noted by the phenol extraction method (Figs. 3,4) and 56 J.
The final eluate was concentrated and centrifuged to equilibrium at 9l,100A for 15 hr through a 25-65% sucrose gradient in NET buffer at lOC. The lower coincident peak of radioactivity, hemagglutinin, and CF activity was then recentrifuged through a 15-65% sucrose velocity gradient for 1 hr at 43,200A. The upper peak of radioactivity and hemagglutinin was used as the starting material for RNA studies. RNA extraction was carried out by five different methods in an effort to find the method best suited for OC-43 RNA.