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By Erik Wischerhoff, Nezha Badi, André Laschewsky, Jean-François Lutz (auth.), Hans G. Börner, Jean-Francois Lutz (eds.)

Erik Wischerhoff, Nezha Badi, André Laschewsky and Jean-François Lutz
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Recently, the Klok group also prepared side-chain PEG-coated surfaces for protein immobilization [201]. They employed surface-initiated ATRP for the synthesis. Terminal hydroxyl groups of the PEGs were activated with p-nitrophenyl chloroformate, and subsequently O6 -benzylguanine was bound. The O6 -benzylguanine-functionalized PEG brushes were used to chemoselectively immobilize O6 -alkylguanine-DNA-alkyltransferase fusion proteins with a defined orientation and surface density. Smart Polymer Surfaces: Concepts and Applications in Biosciences 29 5 Conclusion and Outlook As demonstrated in this chapter, stimuli-responsive polymer surfaces have gained a central role in applied biosciences.

1 Protein-Decorated Surfaces for Spatially Guided Cell Adhesion . . . . . . . . . . 2 Peptide-Decorated Surfaces for Spatially Guided Cell Adhesion. . . . . . . . . . 5 Conclusions and Outlook . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

46 47 56 71 71 1 Surface-Microengineering and Biology: A Challenge at the Interface The progress over the past decades in the field of surface science and the development of several microengineering techniques for the precise deposition, manipulation, and characterization of ultrathin films will open new avenues for the design of novel types of medical devices [1–6]. The new devices will, for example, monitor the health-status of a person for extended time-periods, detect critical metabolic situations such as unusually elevated or strongly reduced levels of blood sugar, or recognize an unexpected, serious immune reaction.

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