Download Atlas of Human Pluripotent Stem Cells in Culture by Lyn Healy, Ludmila Ruban PDF

By Lyn Healy, Ludmila Ruban

This lavishly-illustrated, authoritative atlas explores the elaborate artwork of culturing human pluripotent stem cells. Twelve chapters – containing greater than 280 colour illustrations – hide a number of subject matters in pluripotent stem mobilephone culturing together with mouse and human fibroblasts, human embryonic stem cells and triggered pluripotent stem cells, attribute staining styles, and irregular cultures, between others. Atlas of Human Pluripotent Stem Cells in tradition is a accomplished number of illustrated strategies complemented via informative and academic captions interpreting what high quality cells seem like and the way they behave in quite a few environments. Examples of excellent cultures are in comparison side-by-side to less-than-perfect and unacceptable examples of human embryonic and brought on pluripotent stem mobilephone colonies. This specified and thorough atlas is a useful source for researchers, lecturers, and scholars who're attracted to or operating with stem cellphone culturing.

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Extra info for Atlas of Human Pluripotent Stem Cells in Culture

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2008;52:353–63. 3 Inactivated Mouse and Human Fibroblasts To date, the use of inactivated fibroblasts in the derivation and culture of pluripotent stem cells (PSCs) remains the most widely used culture system despite the availability of other systems that are feeder-free. In general, the feeder-based systems tend to be less expensive than their feeder-free equivalents, and feeders have a proven ability to maintain PSCs a stably in an undifferentiated state, so in most cases the costeffective feeder system is the system of choice.

C) Another good colony similar to the one in a (all ×10 magnification) 4 Human Embryonic Stem Cells Fig. 15 There is spontaneous differentiation (white arrows) on the edges of this colony, but the rest of the colony is undifferentiated (×4 magnification) a Fig. 17 (a) The large colony on the right side is of a relatively good morphology. The smaller colony on the left is not compacted, does not have smooth edges, and the cells are disorganised. Because of these features, the colony is probably going to differentiate within a day or 43 Fig.

These cells would support the growth and expansion of undifferentiated PSCs (a, ×4 magnification; b, ×10) a b c Fig. 18 (a–c) Images of an optimal density mouse feeder culture with a few apoptotic cells present (black arrow) (a, ×4 magnification; b, ×10; c, ×20) 30 3 a b c d Inactivated Mouse and Human Fibroblasts Fig. 19 A comparison between two different cell densities of mouse feeder cells, 3 h after plating. (a, b) Low density. (c, d) High density. Note that the cells have adhered to the culture vessel and are beginning to spread out (a, ×4 magnification; b–d, ×10) 3 Inactivated Mouse and Human Fibroblasts Quality control of feeders should also include an assessment of the ability of a batch of feeders to support the growth and proliferation of undifferentiated PSCs over at least three, preferably five successive passages.

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