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By Minoru Ueda (Ed.)

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B: Three months postoperation. Dome-shaped radiopaque images facing together and extending from the cleft bony walls inside the cavity. C: Six months postoperation. Fused image in the cleft cavity. D: Nine months postoperation. The lateral and supernumerary incisors are approximated in the bony bridge lateral to the reconstructed incisive canal (From Hibi et al. 2006. Reprinted with permission). In the present case the canine coronally forced the mesh plate at 9 months postoperatively, which was earlier than expected.

5 ml of a penicillin–streptomycin mixture containing 25 U of penicillin and 25 µg of streptomycin. The three supplements used for inducing osteogenesis, dexamethasone (Dex), sodium β-glycerophosphate (β-GP) and L-ascorbic acid 2-phosphate (AsAP). The cells were incubated at 37°C in a humidified atmosphere containing 95% air and 5% CO2. 2 ml/cm2 condition medium. The implants were assessed by histological and histomorphometric analysis, 2, 4 and 8 weeks after implantation. The implants exhibited varying degrees of bone-implant contact (BIC).

In particular, the cultured corneal epithelial cell sheet is beneficial for the reconstruction of the ocular surface with limbal stem cell deficiencies [3], such as those with chemical and thermal injuries, ultraviolet and ionizing radiation injuries, Stevens-Johnson syndrome, and ocular cicatricial pemphigoid. Because the sheets can be generated from a small piece of limbal tissue that contains corneal epithelial stem cells [2], cultured corneal epithelial sheet transplantation is less invasive than autolimbal transplantation.

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