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Additional resources for A History of Biochemistry: Selected Topics in the History of Biochemistry Personal Recollections. II.
However, the effect is still evident when mutant phage lacking intact cistrons for nuclease production (gene 46", 47") are used. coli, DNA associated with the RNA-polymerizing system in the bacterial membrane is almost entirely replaced with T4 DNA in a transcription complex . The relatively rapid loss in RNA polymerase activity in the case of bacteriophage T4 but not T5 infection still remains a mystery. However, it must be in some way connected to the beginning of the synthesis of the late functions.
For example, the problem of regulation of biochemical pathways was coming more and more to the forefront. At a conference sponsored by the Ciba Foundation on the Chemistry and Biology of the Purines in London in May of 1956,1 met Donald D. Woods , who was then engaged in an interesting problem of methionine biosynthesis from homocysteine and serine by bacterial extracts of E. coli. One of the components of this system was a protein containing vitamin B 12 as a prosthetic group. During the previous year I had spent a month as a visiting professor at the University of Texas at the invitation of William Shive.
M. BUCHANAN nents . This approach could not reveal, however, the relationship between these proteins and the synthesis of mRNAs from which they were derived. For this reason DNA-RNA competitionhybridization experiments were undertaken by Grasso  in this laboratory and by Salser et al.  to study the synthesis of early T4 mRNAs under a variety of conditions, for example, during inhibition of protein synthesis. When chloramphenicol, an agent that effectively inhibits protein synthesis in prokaryotes, is included at the time of infection of E.